Research

DNA compaction - 03/10/2011

Compaction is the process in which a large DNA molecule undergoes a transition between an elongated conformation and a very compact form. In nature, DNA compaction occurs to package genomic material inside tiny spaces such as viral capsids and cell nuclei. In vitro, several strategies exist to...

Reversible photocontrol of DNA conformation - 03/08/2011

We develop a method to reversibly control DNA conformation using light. It is based on the use of a photosensitive nucleic acd binder (pNAB), which induces DNA compaction in a photodependent manner. As a result, there is pNAB concentration for which DNA folds into compact after blue light...

Photocontrol of gene expression based on light-induced nucleic acid conformational changes - 04/13/2011

Light has been recognized as an ideal external trigger to address gene expression as it offers unique advantages: high spatio-temporal resolution of the excitation, tunability of the intensity, low perturbation of the biochemical environment, biocompatibility, and high potentiality for...

Artificial cell systems - 03/08/2011

Giant vesicles are spherical bags with a phospholipid bilayer membrane and a diameter ranging from 1 to 100 µm. We develop methods to prepare giant vesicles of controlled size and composition. 1) Electroswelling of multilayer phospholipid patterns allows us to get monodisperse unilamellar giant...

Microfluidics for synthetic, cell and systems biology - 04/13/2011

We develop microfluidic devices for applied and fundamental investigations in biology, such as DNA separation, DNA-binder complexes discrimination, immunoassays, on-chip cell culture, sorting and trapping. By constraining yeast cells in curved channels, a novel mechanism linking micro-tubules...

Light-driven microfluidics - 03/11/2011

We develop laser-free methods where a simle light illumination stimulus (e.g., from a LED device) is used to control, manipulate, drive, divide and mix continuous flows (light-driven microfluidics) or discrete droplets (digital optofluidics). 1. Digital optofluidics At the macro-scale, we use...

Article #125 - 12/04/2015


ASAP papers from NATURE
ASAP papers from SCIENCE