16 August 2013. Our manuscript "Cell-free preparation of functional and triggerable giant proteoliposomes " has just been accepted in ChemBioChem.
Abstract: We describe a one-pot, highly robust method to prepare giant liposomes containing functional proteins in their membrane. It consists in the spontaneous transfer of sucrose-in-oil droplets into a cell-free, reconstituted gene expression medium containing a DNA molecule coding for the protein to be inserted in the liposome membrane. We find that the presence of the membrane during gene expression is critical for efficient insertion of the protein into the membrane. Using our method, giant proteoliposomes are formed within 1-3 h without any preliminary protein manipulation or purification step. The reconstituted protein creates functional pores in the membrane that are size-selective. The resulting membrane permeability can also be triggered by a simple temperature stimulus that controls membrane protein expression.
Reference:
Abstract: We describe a one-pot, highly robust method to prepare giant liposomes containing functional proteins in their membrane. It consists in the spontaneous transfer of sucrose-in-oil droplets into a cell-free, reconstituted gene expression medium containing a DNA molecule coding for the protein to be inserted in the liposome membrane. We find that the presence of the membrane during gene expression is critical for efficient insertion of the protein into the membrane. Using our method, giant proteoliposomes are formed within 1-3 h without any preliminary protein manipulation or purification step. The reconstituted protein creates functional pores in the membrane that are size-selective. The resulting membrane permeability can also be triggered by a simple temperature stimulus that controls membrane protein expression.
Reference:
Cell-free preparation of functional and triggerable giant proteoliposomes
Yan-Jun Liu, Gregory P. R. Hansen, Anna Venancio-Marques, and Damien Baigl*
ChemBioChem 2013, 14, 2243 – 2247
- doi : 10.1002/cbic.201300501